Development and Validation of Rp-hplc Method for Simultaneous Determination of Methotrexate, Dexamethasone and Indomethacin
نویسندگان
چکیده
Methods: A simple, isocratic reverse-phase high performance liquid chromatographic (RP-HPLC) method has been developed for simultaneous estimation of methotrexate, dexamethasone and indomethacin using ibuprofen (IBP) as internal standard with photodiode array detection at 254 nm. The process was carried out on C18 column (5 μm, 250 mm X 4.6 mm) using methanol: orthophosphoric acid (1.67 % v/v) in 70: 30 ratio at a flow rate of 1.5 ml per minute.
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